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TeacherTECH Back to School Biotech- DNA Analysis: The Basics of DNA Electrophoresis

Middle School and High School Teachers are invited to attend an exciting fall TeacherTECH workshop series focused on biotech.  Ready to start the new school year? Looking for some fun “out of the box” lab activities to do with your students?  Captivate their imagination. Spark the investigator spirit. Makes the invisible visible and introduce your students to the exciting world of DNA or expand on activities you are already doing in your classes. 

Tuesday, November 30, 2010 

5:00pm - 7:00pm 

San Diego Supercomputer Center, Auditorium

University of California, San Diego 

 

Workshop Description

Use restriction enzyme digestion and DNA gel electrophoresis (popularly known as DNA Fingerprinting) to determine which of a number of suspects could have committed the crime - based on DNA evidence. Extend this kit with a plasmid mapping activity using the plasmid DNA restriction patterns from the experiment. AP Bio Lab 6.

Workshop Activities 

DNA evidence assists in criminal, missing persons, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. Using real DNA as evidence, your students play the role of crime scene investigator to figure out for themselves "Who done it?"

This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis is used to separate and visualize DNA fragments. The unique curriculum provided in this kit guides students through the procedure of constructing a standard curve using their own gel data. They can then use their standard curve to estimate the molecular weights of the unknown DNA fragments generated by different restriction enzymes.
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. Students also have the opportunity to read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the laboratory activity. They can go one step further and use restriction digest maps of lambda bacteriophage genomes (provided in the kit) to design novel plasmids. In the process of doing these extension activities, students learn how restriction enzymes function and how they are used in genetic engineering.

The instructor will also show you materials modifications to fit your class and budget.

Please join us for an exciting and informative session of hands-on learning presented by Essy Levy, Curriculum and Training Specialist, Bio-Rad Laboratories. 

Registration is free. Space is 
limited. Please contact Ange Mason at 858-534-5064 or amason@ucsd.edu to reserve your space.


For additional information on other TeacherTECH programs, please visit http://education.sdsc.edu/teachertech  

 

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